8,499 research outputs found
The interplay between tissue growth and scaffold degradation in engineered tissue constructs
In vitro tissue engineering is emerging as a potential tool to meet the high demand for replacement tissue, caused by the increased incidence of tissue degeneration and damage. A key challenge in this field is ensuring that the mechanical properties of the engineered tissue are appropriate for the in vivo environment. Achieving this goal will require detailed understanding of the interplay between cell proliferation, extracellular matrix (ECM) deposition and scaffold degradation.\ud
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In this paper, we use a mathematical model (based upon a multiphase continuum framework) to investigate the interplay between tissue growth and scaffold degradation during tissue construct evolution in vitro. Our model accommodates a cell population and culture medium, modelled as viscous fluids, together with a porous scaffold and ECM deposited by the cells, represented as rigid porous materials. We focus on tissue growth within a perfusion bioreactor system, and investigate how the predicted tissue composition is altered under the influence of (i) differential interactions between cells and the supporting scaffold and their associated ECM, (ii) scaffold degradation, and (iii) mechanotransduction-regulated cell proliferation and ECM deposition.\ud
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Numerical simulation of the model equations reveals that scaffold heterogeneity typical of that obtained from μCT scans of tissue engineering scaffolds can lead to significant variation in the flow-induced mechanical stimuli experienced by cells seeded in the scaffold. This leads to strong heterogeneity in the deposition of ECM. Furthermore, preferential adherence of cells to the ECM in favour of the artificial scaffold appears to have no significant influence on the eventual construct composition; adherence of cells to these supporting structures does, however, lead to cell and ECM distributions which mimic and exaggerate the heterogeneity of the underlying scaffold. Such phenomena have important ramifications for the mechanical integrity of engineered tissue constructs and their suitability for implantation in vivo
Pragmatic Ontology Evolution: Reconciling User Requirements and Application Performance
Increasingly, organizations are adopting ontologies to describe their large catalogues of items. These ontologies need to evolve regularly in response to changes in the domain and the emergence of new requirements. An important step of this process is the selection of candidate concepts to include in the new version of the ontology. This operation needs to take into account a variety of factors and in particular reconcile user requirements and application performance. Current ontology evolution methods focus either on ranking concepts according to their relevance or on preserving compatibility with existing applications. However, they do not take in consideration the impact of the ontology evolution process on the performance of computational tasks – e.g., in this work we focus on instance tagging, similarity computation, generation of recommendations, and data clustering. In this paper, we propose the Pragmatic Ontology Evolution (POE) framework, a novel approach for selecting from a group of candidates a set of concepts able to produce a new version of a given ontology that i) is consistent with the a set of user requirements (e.g., max number of concepts in the ontology), ii) is parametrised with respect to a number of dimensions (e.g., topological considerations), and iii) effectively supports relevant computational tasks. Our approach also supports users in navigating the space of possible solutions by showing how certain choices, such as limiting the number of concepts or privileging trendy concepts rather than historical ones, would reflect on the application performance. An evaluation of POE on the real-world scenario of the evolving Springer Nature taxonomy for editorial classification yielded excellent results, demonstrating a significant improvement over alternative approaches
Maternal and Gestational Influences on Deciduous and Permanent Tooth Size
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66608/2/10.1177_00220345800590021301.pd
Timing and causes of North African wet phases during the last glacial period and implications for modern human migration
We present the first speleothem-derived central North Africa rainfall record for the last glacial period. The record reveals three main wet periods at 65-61 ka, 52.5-50.5 ka and 37.5-33 ka that lead obliquity maxima and precession minima. We find additional minor wet episodes that are synchronous with Greenland interstadials. Our results demonstrate that sub-tropical hydrology is forced by both orbital cyclicity and North Atlantic moisture sources. The record shows that after the end of a Saharan wet phase around 70 ka ago, North Africa continued to intermittently receive substantially more rainfall than today, resulting in favourable environmental conditions for modern human expansion. The encounter and subsequent mixture of Neanderthals and modern humans – which, on genetic evidence, is considered to have occurred between 60 and 50 ka – occurred synchronously with the wet phase between 52.5 and 50.5 ka. Based on genetic evidence the dispersal of modern humans into Eurasia started less than 55 ka ago. This may have been initiated by dry conditions that prevailed in North Africa after 50.5 ka. The timing of a migration reversal of modern humans from Eurasia into North Africa is suggested to be coincident with the wet period between 37.5 and 33 ka
The dose makes the poison: have “field realistic” rates of exposure of bees to neonicotinoid insecticides been overestimated in laboratory studies?
Recent laboratory based studies have demonstrated adverse sub-lethal effects of neonicotinoid insecticides on honey bees and bumble bees, and these studies have been influential in leading to a European Union moratorium on the use of three neonicotinoids, clothianidin, imidacloprid, and thiamethoxam on “bee attractive” crops. Yet so far, these same effects have not been observed in field studies. Here we review the three key dosage factors (concentration, duration and choice) relevant to field conditions, and conclude that these have probably been over estimated in many laboratory based studies
Long quantum channels for high-quality entanglement transfer
High-quality quantum-state and entanglement transfer can be achieved in an
unmodulated spin bus operating in the ballistic regime, which occurs when the
endpoint qubits A and B are coupled to the chain by an exchange interaction
comparable with the intrachain exchange. Indeed, the transition amplitude
characterizing the transfer quality exhibits a maximum for a finite optimal
value , where is the channel length. We show that
scales as for large and that it ensures a
high-quality entanglement transfer even in the limit of arbitrarily long
channels, almost independently of the channel initialization. For instance, the
average quantum-state transmission fidelity exceeds 90% for any chain length.
We emphasize that, taking the reverse point of view, should be
experimentally constrained, high-quality transfer can still be obtained by
adjusting the channel length to its optimal value.Comment: 12 pages, 9 figure
The SSS phase of RS Ophiuchi observed with Chandra and XMM-Newton I.: Data and preliminary Modeling
The phase of Super-Soft-Source (SSS) emission of the sixth recorded outburst
of the recurrent nova RS Oph was observed twice with Chandra and once with
XMM-Newton. The observations were taken on days 39.7, 54.0, and 66.9 after
outburst. We confirm a 35-sec period on day 54.0 and found that it originates
from the SSS emission and not from the shock. We discus the bound-free
absorption by neutral elements in the line of sight, resonance absorption lines
plus self-absorbed emission line components, collisionally excited emission
lines from the shock, He-like intersystem lines, and spectral changes during an
episode of high-amplitude variability. We find a decrease of the oxygen K-shell
absorption edge that can be explained by photoionization of oxygen. The
absorption component has average velocities of -1286+-267 km/s on day 39.7 and
of -771+-65 km/s on day 66.9. The wavelengths of the emission line components
are consistent with their rest wavelengths as confirmed by measurements of
non-self absorbed He-like intersystem lines. We have evidence that these lines
originate from the shock rather than the outer layers of the outflow and may be
photoexcited in addition to collisional excitations. We found collisionally
excited emission lines that are fading at wavelengths shorter than 15A that
originate from the radiatively cooling shock. On day 39.5 we find a systematic
blue shift of -526+-114 km/s from these lines. We found anomalous He-like f/i
ratios which indicates either high densities or significant UV radiation near
the plasma where the emission lines are formed. During the phase of strong
variability the spectral hardness light curve overlies the total light curve
when shifted by 1000sec. This can be explained by photoionization of neutral
oxygen in the line of sight if the densities of order 10^{10}-10^{11} cm^{-3}.Comment: 16 pages, 10 figures, 4 tables. Accepted by ApJ; v2: Co-author
Woodward adde
Freak Waves in Random Oceanic Sea States
Freak waves are very large, rare events in a random ocean wave train. Here we
study the numerical generation of freak waves in a random sea state
characterized by the JONSWAP power spectrum. We assume, to cubic order in
nonlinearity, that the wave dynamics are governed by the nonlinear Schroedinger
(NLS) equation. We identify two parameters in the power spectrum that control
the nonlinear dynamics: the Phillips parameter and the enhancement
coefficient . We discuss how freak waves in a random sea state are more
likely to occur for large values of and . Our results are
supported by extensive numerical simulations of the NLS equation with random
initial conditions. Comparison with linear simulations are also reported.Comment: 7 pages, 6 figures, to be published in Phys. Rev. Let
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